 | Robert Lowery
Batman |
 | Johnny Duncan
Robin |
 | Jane Adams
Vicki Vale |
 | Lyle Talbot
Commissioner Jim Gordon |
 | Ralph Graves
Winslow Harrison [Chs. 7-8] |
 | Don C. Harvey
Henchman Nolan [Chs. 1-6] |
 | William Fawcett
Prof. Hammil |
 | Leonard Penn
Carter - Hammil's Valet |
 | Rick Vallin
Barry Brown |
 | Michael Whalen
Private Investigator Dunne [Chs. 2, 9, 12] |
| Greg McClure
Henchman Evans [Chs. 1-6] |
 | House Peters Jr.
Henchman Earl [Chs. 7-15] |
 | Jim Diehl
Henchman Jason [Chs. 3-15] |
 | Rusty Wescoatt
Henchman Ives [Chs. 3-13] |
 | Eric Wilton
Alfred Pennyworth |
 | Phil Arnold
Doctor [Ch. 4] |
| Marshall Bradford
Wesley Morton [Chs. 3-4] |
 | Jack Chefe
Plant Guard in Hallway [Chs. 13-14] |
 | George Cisar
Policeman in Alley [Ch. 1] |
 | Richard Cramer
Newspaper Office Worker [Ch. 7] (archive footage) |
| James Craven
Norwood [Chs. 1, 3, 12-14] |
 | John Doucette
Henchman [Ch. 2, 3, 6, 10-12, 15] |
 | Frank Ellis
Policeman Assigned to Set Up Jason's Escape [Ch. 10] |
 | Frank Hagney
Plant Guard [Ch. 14] |
 | John Hart
John Hench / Mechanic [Ch. 2] |
 | Myron Healey
Walker, Policeman [Ch. 7] |
 | Knox Manning
Narrator (voice) |
 | Frank O'Connor
Plant Gate Guard [Chs. 13-14] |
 | George Offerman Jr.
Henchman Jimmy Vale [Chs. 1-2, 5, 8-11] |
 | Eddie Parker
Holt [Chs. 1, 5-8, 12, 15] |
| Allan Ray
Mac Lacey [Ch. 5] |
| Lee Roberts
Henchman Neal [Chs. 7-15] |
 | George Robotham
Car 4 Henchman-Driver [Chs. 2-6, 11-12] |
 | Emmett Vogan
Mr. Williams [Chs. 1, 3, 12-14] |
 | Wally West
Pursuit Cop [Ch. 15] |