 | Robert Lowery Batman |
 | Johnny Duncan Robin |
 | Jane Adams Vicki Vale |
 | Lyle Talbot Commissioner Jim Gordon |
 | Ralph Graves Winslow Harrison [Chs. 7-8] |
 | Don C. Harvey Henchman Nolan [Chs. 1-6] |
 | William Fawcett Prof. Hammil |
 | Leonard Penn Carter - Hammil's Valet |
 | Rick Vallin Barry Brown |
 | Michael Whalen Private Investigator Dunne [Chs. 2, 9, 12] |
| Greg McClure Henchman Evans [Chs. 1-6] |
 | House Peters Jr. Henchman Earl [Chs. 7-15] |
 | Jim Diehl Henchman Jason [Chs. 3-15] |
 | Rusty Wescoatt Henchman Ives [Chs. 3-13] |
 | Eric Wilton Alfred Pennyworth |
 | Phil Arnold Doctor [Ch. 4] |
| Marshall Bradford Wesley Morton [Chs. 3-4] |
 | Jack Chefe Plant Guard in Hallway [Chs. 13-14] |
 | George Cisar Policeman in Alley [Ch. 1] |
 | Richard Cramer Newspaper Office Worker [Ch. 7] (archive footage) |
| James Craven Norwood [Chs. 1, 3, 12-14] |
 | John Doucette Henchman [Ch. 2, 3, 6, 10-12, 15] |
 | Frank Ellis Policeman Assigned to Set Up Jason's Escape [Ch. 10] |
 | Frank Hagney Plant Guard [Ch. 14] |
 | John Hart John Hench / Mechanic [Ch. 2] |
 | Myron Healey Walker, Policeman [Ch. 7] |
 | Knox Manning Narrator (voice) |
 | Frank O'Connor Plant Gate Guard [Chs. 13-14] |
 | George Offerman Jr. Henchman Jimmy Vale [Chs. 1-2, 5, 8-11] |
 | Eddie Parker Holt [Chs. 1, 5-8, 12, 15] |
| Allan Ray Mac Lacey [Ch. 5] |
| Lee Roberts Henchman Neal [Chs. 7-15] |
 | George Robotham Car 4 Henchman-Driver [Chs. 2-6, 11-12] |
 | Emmett Vogan Mr. Williams [Chs. 1, 3, 12-14] |
 | Wally West Pursuit Cop [Ch. 15] |